Study of the Immunological Pathophysiological Mechanisms Associated With Acute Respiratory Distress Syndrome
Objectives: To characterize the inflammatory profile of patients with ARDS using circulating biomarkers and single-cell RNA sequencing of pulmonary immune cells. The investigators hypothesize that there is a correlation between the profile of serum biomarkers (inflammatory sub-phenotypes), the transcriptome of pulmonary immune cells. Briefly the experimental scheme is as follow: * Population: patients with ARDS under invasive mechanical ventilation in the ICU. * Intervention: 1. Determination of the inflammatory subphenotype on circulatory inflammatory biomarkers. 2. Characterization of inflammation by single cell RNA sequencing on lung immune cells collected on broncho-alveolar fluid.
• ARDS risk factors: bacterial or viral pneumonia, extrapulmonary infection, major trauma, transfusion, inhalation injury, or shock.
• Pulmonary edema not explained by a cardiogenic cause or volume overload.
• Onset of respiratory symptoms within \<7 days.
• Bilateral pulmonary involvement on chest X-ray, CT scan, or ultrasound.
• PaO₂/FiO₂ ≤ 300 assessed with PEEP ≥ 5 cmH₂O.