• Histologically-confirmed esophageal squamous cell carcinoma and whose tissue samples were taken before treatment;

• Tumors of the esophagus are located in the thoracic cavity;

• Pre-treatment stage as clinical II-III (AJCC/UICC 8th Edition)

• Age is between 18 years and 75 years;

• Eastern Cooperative Oncology Group (ECOG) performance status 0-1, and expected survival time ≥12 months;

• Adequate cardiac function. All patients should perform ECG, and those with a cardiac history or ECG abnormality should perform echocardiography with the left ventricular ejection fraction \> 50 %;

• Adequate respiratory function with FEV1≥1.2L, FEV1%≥50% and DLCO≥50% shown in pulmonary function tests ;

• Adequate bone marrow function (White Blood Cells \>4x10\^9 /L; Neutrophil \>2.0×10\^9 /L; Hemoglobin \> 90 g/L; platelets\>100x10\^9 /L);

• Adequate liver function (Total bilirubin \<1.5x Upper Level of Normal (ULN); Aspartate transaminase(AST) and Alanine transaminase (ALT) \<1.5x ULN);

⁃ Adequate renal function (Glomerular filtration rate (CCr) \>60 ml/min; serum creatinine (SCr) ≤120 µmol/L);

⁃ The patient has provided written informed consent and is able to understand and comply with the study;