• Patients with melanoma have received standard first-line therapy and have been diagnosed with non-resectable, metastatic, progressive or recurrent conditions.

• The expression of melanoma specific antigens is immunohistochemically stained and verified.

• Body weight greater than or equal to 40 kg.

• Age: ≥18 year and ≤ 75 years of age at the time of enrollment.

• Life expectancy: at least 8 weeks.

• Prior Therapy:

‣ There is no limit to the number of prior treatment regimens. Any grade 3 or 4 non-hematologic toxicity of any previous therapy must be resolved to grade 2 or less.

⁃ Participants must not have received hematopoietic growth factors for at least 1 week prior to mononuclear cells collection.

⁃ At least 7 days must have elapsed since the completion of therapy with any biologic agent, targeted agent, tyrosine kinase inhibitor or metronomic non-myelosuppressive regimen.

⁃ At least 4 weeks must have elapsed since prior therapy that includes a monoclonal antibody.

⁃ At least 1 week must has elapsed since any radiation therapy at the time of study entry.

• Karnofsky/jansky score of 70% or greater.

• Cardiac function: Left ventricular ejection fraction greater than or equal to 40/55 percent.

• Pulse Ox greater than or equal to 90% on room air.

• Liver function: defined as alanine transaminase (ALT) \<3x upper limit of normal (ULN), aspartate aminotransferase (AST) \<3x ULN; serum bilirubin and alkaline phosphatase \<2x ULN.

• Renal function: Patients must have serum creatinine less than 3 times ULN.

• Marrow function: White blood cell count ≥1000/ul, Absolute neutrophil count ≥500/ul, Absolute lymphocyte count ≥500/ul, Platelet count ≥25,000/ul (not achieved by transfusion).

• Patients with known bone marrow metastatic disease will be eligible for study as long as they meet hematologic function criteria, and the marrow disease not evaluable for hematologic toxicity.

• For all patients enrolled in this study, the patients or their legal guardians must sign an informed consent and assent.