‣ De novo type 1 diabetic patient, as per ISPAD criteria;

⁃ Symptoms of hyperglycemia: polyuria-polydipsia-amaigrin +/- Acido ketosis.

⁃ Fasting blood glucose ≥126 mg/dL AND/OR blood glucose ≥200 mg/dL at 120 minutes of an OGTT AND/OR HbA1c ≥6.5% AND/OR a patient with symptoms of hyperglycemia/hyperglycemic crisis (see 8. a. 2.) with random blood glucose ≥200 mg/dL.

∙ Presence in serum of one or more anti-islet autoantibodies (anti-insulin, anti-IA2, anti-GAD65, anti-ZnT8)

• Patients aged between 2 and 30 years

• Minimum weight: 17 kg (for blood samples)

• Male - female patients

• Free, written and oral consent.

‣ De novo type 1 diabetic patient, as per ISPAD criteria;

⁃ Symptoms of hyperglycemia: polyuria-polydipsia-amaigrin +/- Acido ketosis.

⁃ Fasting blood glucose ≥126 mg/dL AND/OR blood glucose ≥200 mg/dL at 120 minutes of an OGTT AND/OR HbA1c ≥6.5% AND/OR a patient with symptoms of hyperglycemia/hyperglycemic crisis (see 8. a. 2.) with random blood glucose ≥200 mg/dL.

⁃ Presence in serum of one or more anti-islet autoantibodies (anti-insulin, anti-IA2, anti-GAD65, anti-ZnT8)

⁃ Patients aged between 2 years and 18 years (\<18 years).

⁃ Male - female patients

⁃ Free, written and oral consent.

‣ Adult older than 18 years.

⁃ Absence of blood marker of diabetes (Absence of antibodies, HbA1C \<6.5%, C-peptide \> 0.18 nmol/L, Fasting blood glucose \< 100 mg/dL, blood glucose at any time \< 200 mg/dL).

⁃ Be a first-degree relative with a patient being followed for diabetes (meeting ISPAD criteria).

⁃ Male - Female

⁃ Free written and oral consent

∙ Cohort of patients followed for cystic fibrosis:

• Pediatric patient between 2 and 18 years of age.

• Diagnosed with cystic fibrosis with impaired pancreatic endocrine function.

• Presents glucose homeostasis disorders (regular hypo/hyper-glycemia).

• Male - female patient

• Free, written and oral consent

∙ Cohort of patients with (sub)total pancreatectomy:

• Pediatric patients between 2 and 18 years of age.

• Follow-up for total pancreatectomy or caudal pancreatectomy

• Presents disorders of carbohydrate homeostasis (regular hypo-/hyper-glycemia)

• Male - female patient

• Free, written and oral consent

‣ Patient who has undergone insulin testing due to suspected growth hormone deficiency or adrenal insufficiency or hypopituitarism.

⁃ Patients between the ages of 2 years and 18 years (\<18 years).

⁃ Male - female patient.

⁃ Free written and oral consent.

‣ Type 1 diabetic patient, as per ISPAD criteria;

⁃ Symptoms of hyperglycemia: polyuria-polydipsia-amaigrin +/- Acido ketosis.

⁃ Fasting blood glucose ≥126 mg/dL AND/OR blood glucose ≥200 mg/dL at 120 minutes of an OGTT AND/OR HbA1c ≥6.5% AND/OR a patient with symptoms of hyperglycemia/hyperglycemic crisis (see 8. a. 2.) with random blood glucose ≥200 mg/dL.

⁃ Presence in serum of one or more anti-islet autoantibodies (anti-insulin, anti-IA2, anti-GAD65, anti-ZnT8)

⁃ Patients aged between 2 and 18 years (\<18 years).

⁃ Male - female patients

⁃ Free, written and oral consent.

‣ De novo type 1 diabetic patient, as per ISPAD criteria;

⁃ Symptoms of hyperglycemia: polyuria-polydipsia-amaigrin +/- Acido ketosis.

⁃ Fasting blood glucose ≥126 mg/dL AND/OR blood glucose ≥200 mg/dL at 120 minutes of an OGTT AND/OR HbA1c ≥6.5% AND/OR a patient with symptoms of hyperglycemia/hyperglycemic crisis (see 8. a. 2.) with random blood glucose ≥200 mg/dL.

⁃ Presence in serum of one or more anti-islet autoantibodies (anti-insulin, anti-IA2, anti-GAD65, anti-ZnT8)

⁃ Patients aged between 2 and 18 years

⁃ Minimum weight: 17 kg (for blood samples)

⁃ Male - female patients

⁃ Free, written and oral consent.