Development of a Protocol for Non-Invasive Prenatal Analysis of Cell-Free Circulating Fetal DNA (ccfDNA) Using Massively Parallel Sequencing (MPS)
In criminal cases involving sexual assault, victims may later discover they are pregnant, creating profound uncertainty about the paternity of the fetus. These distressing situations compound the trauma already experienced by the victims. To address this, the Medico-Legal Hematology Laboratory (LHML) in Bordeaux aims to validate a method based on Massively Parallel Sequencing (MPS) and a specific isolation protocol for analyzing cell-free circulating fetal DNA (ccfDNA) in maternal blood samples. This identification process will confirm or exclude paternal genetic relationships between the fetus and potential fathers between 6 and 22 weeks of amenorrhea. These analyses will only be conducted under judicial requisition, in accordance with the French Code of Criminal Procedure
• Age 18 or older.
• For woman: pregnancy between 6 and 22 weeks
• For both mother and father: non-opposition to participate and genetic signed informed consent