Investigation of Salivary E-cadherin, Calprotectin, and Matrix Metalloproteinase-9 Levels in Patients With Recurrent Aphthous Stomatitis

Status: Recruiting
Location: See location...
Intervention Type: Diagnostic test
Study Type: Observational
SUMMARY

The goal of this observational cross-sectional study is to determine the salivary levels of E-cadherin, Calprotectin, and Matrix Metalloproteinase-9 (MMP-9) in patients with Recurrent Aphthous Stomatitis (RAS) and to compare them with those of healthy controls. The main questions it aims to answer are: Are the salivary levels of E-cadherin, Calprotectin, and MMP-9 significantly different in patients with minor RAS compared to healthy controls? Can these salivary proteins serve as biomarkers involved in the pathogenesis of minor RAS? Researchers will compare patients with minor RAS and healthy individuals to determine whether differences in salivary E-cadherin, Calprotectin, and MMP-9 levels are associated with the presence of disease. Participants will: Provide unstimulated saliva samples using a saliva swab Undergo clinical evaluation for periodontal status (Community Periodontal Index - CPI) and caries experience (DMFT index) Collected saliva samples will be stored at -80°C until analysis. The concentrations of the investigated proteins will be measured using ELISA kits, and total protein levels will be determined using a colorimetric protein assay. The relationships among the salivary concentrations of the investigated proteins, as well as their associations with demographic variables such as age and sex, will be analyzed.

Eligibility
Participation Requirements
Sex: All
Minimum Age: 18
Healthy Volunteers: t
View:

• Participants aged 18 years or older

• Clinically diagnosed with minor recurrent aphthous stomatitis (RAS) (defined as having a history of at least three RAS episodes within the past year)

• Systemically healthy (i.e., without any diagnosed systemic disease)

• No history of tobacco use

• Presence of at least one active aphthous ulcer at the time of saliva sample collection

• No known mental or physical disabilities

Locations
Other Locations
Turkey
Aydın Adnan Menderes University
RECRUITING
Aydin
Contact Information
Primary
Sunay Hoşgör, dds
sunayhosgor@gmail.com
+905313636708
Backup
Selin Yeşiltepe, dds,msd
dt_selin@yahoo.com
+905058282990
Time Frame
Start Date: 2025-04-10
Estimated Completion Date: 2026-06-30
Participants
Target number of participants: 102
Treatments
Minor Recurrent Aphthous Stomatitis Patients
Participants aged 18 years or older who have been clinically diagnosed with minor recurrent aphthous stomatitis (RAS) based on characteristic clinical features and medical history. The lesion must be round or oval, less than 5 mm in diameter, surrounded by an erythematous halo, and covered by a grayish-white pseudomembrane. Eligible patients must have experienced at least three episodes of RAS within the past year and present with at least one aphthous ulcer at the time of clinical examination and saliva collection.
Healthy Controls
Participants aged 18 years or older with no lifetime history of recurrent aphthous stomatitis. Control group participants will be selected to match the patient group in terms of age and gender distribution. At the time of saliva collection, no aphthous lesions or any active oral mucosal pathology should be present.
Related Therapeutic Areas
Sponsors
Collaborators: Aydin Adnan Menderes University, Aydın Adnan Menderes University Scientific Research Projects Unit
Leads: Selin Yeşiltepe

This content was sourced from clinicaltrials.gov