• 18≤Age≤80 years;

• First stroke or complete self-care before the onset of current stroke (mRS 0-1);

• Administration of drugs can be completed within 24h of the onset of signs and symptoms of neurological deficits (in patients with wake-up strokes or unwitnessed strokes, the time of onset is defined as the time of last normal presentation);

• Clinical symptoms, signs and imaging diagnosis of cerebral infarction in the area supplied by the middle cerebral artery, together with the following features:

‣ 16≤ NIHSS ≤32 points, and the sum of the scores of motor arm and motor leg is ≥6;

⁃ Imaging suggestive of core infarct area: apparent diffusion coefficient (ADC) values \<620×10\^-6mm\^2/s lesion volume on magnetic resonance imaging (MRI) diffusion-weighted imaging (DWI) sequences or cerebral blood flow (CBF) \<30% of volume 70-300 ml on computed tomography perfusion imaging (CTP) or an Alberta Stroke Program Early CT Score (ASPECTS) of 0-6, with inconsistent findings, the investigator is required to make a reasoned judgement by taking into account all the information (scanning time, the imaging method that best responds to the size of infarct, etc.) and record it.

• NIHSS score not improving or progressing after reperfusion therapy and total score still ≤32.

• Informed consent signed