Exploring Biomarkers for Therapeutic Response of Lupus Nephritis Based on Multi Omics
This is an observational study of patients with lupus nephritis aiming to find biomarkers that can predict patients' response to immunosuppressants. We planed to collect 100 lupus nephritis patients' peripheral blood,kidney tissues and urine before and after treatment (mycophenolate mofetil or cyclophosphamide, in combination with glucocorticoids). Then multi omics analysis, including single cell RNA-seq, ATAC-seq and CITE-seq, will be performed to find new biomarkers for patients' response and prognosis.
• Age between 16-60 years.
• Fulfilling the 2012 SLICC classification for SLE.
• Renal biopsy pathology was clearly classified as III \ IV \ V+III \ V+IV within 6 months.
• Renal biopsy reveals active disease.
• 24-hour urinary protein was tested twice within two weeks, both of which were greater than 1g.
• The patients have never received glucocorticoid/immunosuppressants or received standard treatment for more than 1 month without change. The standard treatment should fulfill: a. prednisone 0-20mg/day, or equivalent other glucocorticoids; b. acceptable immunosuppressants, including Tacrolimus ≤ 4mg/d, methotrexate ≤ 15mg/week, azathioprine ≤ 100mg/d and MMF ≤ 1g/d.
• Informed consent obtained.