Transcriptomic Profiling of Cumulus Cells From CAPA-IVM Cycles in Women With PCOS: A Prospective Pilot Study

Status: Recruiting
Location: See location...
Study Type: Observational
SUMMARY

Capacitation in vitro maturation (CAPA-IVM) has improved oocyte maturation and resulted in live births. Because cumulus cells (CCs) communicate bidirectionally with the oocyte, their transcriptomic profile may serve as a non-invasive biomarker of oocyte and embryo competence. This prospective pilot study will analyze CCs gene expression after CAPA-IVM using RNA sequencing and pathway analysis, and correlate findings with embryological outcomes including fertilization, day-3 cleavage, and blastocyst formation. Results are expected to provide insights into the molecular basis of oocyte competence and support development of non-invasive embryo selection strategies.

Eligibility
Participation Requirements
Sex: Female
Minimum Age: 18
Maximum Age: 38
Healthy Volunteers: f
View:

• Women 18-38 years

• BMI =\< 32kg/m2

• Diagnosed with polycystic ovary syndrome (PCOS) according to the Rotterdam 2003 criteria, meet at least 2 of the following 3 features: (1) Oligo-ovulation or anovulation. (2) Clinical and/or biochemical signs of hyperandrogenism (3) Polycystic ovarian morphology on ultrasound.

• Indicated for CAPA-IVM treatment.

• Serum AMH ≥ 4 ng/mL (28.57 pmol/L).

• Antral follicle count (AFC) ≥ 24 follicles in both ovaries at the time of CAPA-IVM indication.

• Signed informed consent to participate in the study.

Locations
Other Locations
Viet Nam
My Duc Hospital
RECRUITING
Ho Chi Minh City
Contact Information
Primary
Huy H Pham, MSc.
huy.ph@myduchospital.vn
+84966145510
Time Frame
Start Date: 2025-10-16
Estimated Completion Date: 2026-03-30
Participants
Target number of participants: 10
Treatments
PCOS Women Undergoing CAPA-IVM
Women aged 18-38 years with PCOS (Rotterdam criteria) indicated for CAPA-IVM. From each participant, 15 cumulus-oocyte complexes (COCs) are allocated to single-culture CAPA-IVM. Cumulus cells from these COCs are collected at oocyte denudation for RNA sequencing and transcriptomic analysis.
Sponsors
Leads: Mỹ Đức Hospital
Collaborators: Vrije Universiteit Brussel

This content was sourced from clinicaltrials.gov

Similar Clinical Trials