MIRSEP - MIRNA QUANTIFICATION IN PATIENTS WITH SEPTIC SHOCK
Sepsis-induced immunosuppression (SIS) is a common complication in patients with septic shock. Reduced expression of Human leukocyte antigen isotype DR (HLA-DR) on circulating monocytes is a marker for SIS and correlates with risk of secondary infections and mortality. The miRSep study aims to improve the understanding of early microRNA (miRNA) mediated changes in HLA-DR on monocytes in patients with septic shock.
⁃ Septic shock:
• New onset (\<24h) of septic shock diagnosis according to Sepsis-3 definition
• (suspected) infection
• Vasopressors required to maintain mean arterial pressure ≥65mm Hg (despite adequate fluid resuscitation)
• Serum lactate level \> 2mmol/L
• Minimum age of 18 years
• Expected length of stay \>48h
• Written consent from an independent physician
⁃ Critically ill:
• Patients on mechanical ventilation (MV) in the ICU without an admission diagnosis of sepsis/septic shock according to Sepsis-3 definition
• Minimum age of 18 years
• Expected length of stay \>48h
• Written consent from an independent physician