• Female aged 18 years or older

• Biopsy-proven vulvar lichen sclerosus lesion

• Experiencing one or more of the following symptoms of LS:

‣ Dryness

⁃ Itching

⁃ Burning

⁃ Bleeding

⁃ Blistering

⁃ Soreness

⁃ Easily bruises

⁃ Easily tears

⁃ Ulcerated lesions

‣ Painful intercourse

• Negative urine pregnancy test if subject is of childbearing potential before enrollment

• Absence of vulvovaginal infection i.e. fungi, bacterial vaginosis, and STI

• Ability to understand and sign informed consent, questionnaires, and all investigation requirements

• Willing to consent to clinical photographs of the treatment area

• Willing to consent to ultrasound images of the treatment area

• Willing and able to logistically follow schedule of treatments and follow-up visits