Observational Study to Investigate the Correlation of the Genetic Constitution of the Spent Blastocyst Media (SBM) with the Inner Cell Mass (ICM) and Multifocal Trophectoderm (TE) Biopsies
The main objective of this study is to improve niPGT-A (non-invasive Preimplantation Genetic Testing for Aneuploidies) to avoid the need of a biopsy to perform the genetic analysis of the embryo and to help in understanding the genetic constitution of different parts of the blastocyst (early stage of an embryo development). For this purpose, we aim to analyses the genetic material released by the embryo to the culture medium (also called Spent Blastocyst Media, SBM) from about 200 blastocysts donated to research by participants of assisted reproduction treatments. The results will be correlated with the ones obtained from performing the same analysis on biopsies taken from the outer (trophectoderm, TE) and the inner (inner cell mass, ICM) layer of cells in different locations to investigate the mechanisms and origin of the blastocyst genetic content released to the culture medium during preimplantation embryo development.
• Study informed consent form, approved by the Ethics Committee, signed.
• Any patient with aneuploid or euploid blastocysts from PGT-A cycles donated for research.
• Patients undergoing regular IVF/ICSI cycles aiming to donate surplus embryos for research.
• Embryos from own or donated oocytes can be included.
• Embryos fertilized with donated sperm can be included.
• Only blastocysts with proper re-expansion and good quality after thawing will be included.