• Subject is a male or female 18 years of age or older.

• Subject is undergoing planned resection of known or suspected GBM.

• Subject has a Karnofsky Performance Status (KPS) 70% or greater.

• Subject has a life expectancy of at least 6 months, in the opinion of the Investigator.

• Based on the pre-operative evaluation by neurosurgeon, the subject is a candidate for ≥ 80% resection of enhancing region.

• Subject must be able to undergo MRI evaluation.

• Subject meets the following laboratory criteria:

∙ White blood count ≥ 3,000/μL

‣ Absolute neutrophil count ≥ 1,500/μL

‣ Platelets ≥ 100,000/μL

‣ Hemoglobin \> 10.0 g/dL (transfusion and/or ESA allowed)

‣ Total bilirubin and alkaline phosphatase ≤ 2x institutional upper limit of normal (ULN)

‣ Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) \< 3 x ULN

‣ Blood urea nitrogen (BUN) and creatinine \< 1.5 x ULN

• Females of reproductive potential must have a negative serum pregnancy test and be willing to use an acceptable method of birth control.

• Males of reproductive potential must be willing to use an acceptable method of birth control to ensure effective contraception with partner.

⁃ Able to understand and willing to sign an institutional review board (IRB)- approved written informed consent document (legally authorized representative permitted).

• Subject has a histologically confirmed (frozen section) diagnosis of WHO Grade IV glioblastoma multiforme (GBM).

• TPFF and/or pericranial flap is technically feasible.